Introduction
ºûÀÌ ½Ã·á¸¦ Åë°úÇϰųª ¹Ý»çµÉ ¶§ ½Ã·á¿¡ Èí¼öµÇ´Â ºûÀÇ ¾çÀº ÀԻ籤 (Incident Radiation, I¡Æ)°ú Åõ°ú±¤ (Transmitted Radiation, I)ÀÇ Â÷ÀÌ´Ù.
Èí¼öµÈ ºûÀÇ ¾çÀº Åõ±¤µµ ȤÀº Èí±¤µµ·Î Ç¥ÇöµÈ´Ù.
Åõ±¤µµ (T)´Â ÀԻ籤¿¡ ´ëÇÑ Åõ°ú±¤ÀÇ ºñÀ²À» ¸»Çϸç, ´ÙÀ½°ú °°ÀÌ Á¤ÀǵȴÙ.
T = I / I¡Æ ¶Ç´Â %T = (I / I¡Æ) x 100
Åõ±¤µµ ¿ª¼öÀÇ »ó¿ë´ë¼ö¸¦ Èí±¤µµ (A)¶ó°í Á¤ÀÇÇÑ´Ù.
A = -logT
À̹ø ½ÇÇè¿¡¼´Â, À¯¸®ÆÇÀÇ Åõ±¤µµ¿Í IR FilterÀÇ Wavelength Cutoff¸¦ ÃøÁ¤ÇÏ¿´´Ù.
PDA (Photodiode Array) ¹æ½ÄÀÇ UV-Vis Spectrophotometer´Â IR Filter¿¡¼ ºûÀÇ Á¤È®ÇÑ Cutoff¸¦ È®ÀÎÇÒ ¶§ »ç¿ëµÉ ¼ö ÀÖ´Ù. À̹ø ÀÀ¿ë¿¡¼Ã³·³, IR FilterÀÇ Quality¸¦ Æò°¡ÇÏ·Á¸é Àü ÆÄÀå ½ºÆåÆ®·³À» ¾ò¾î¾ß Çϴµ¥, PDA UV-Vis Spectrophotometer¸¦ ÀÌ¿ëÇÏ¿© Àü ÆÄÀåÀÇ ½ºÆåÆ®·³À» µ¿½Ã¿¡ ÃøÁ¤Çϸé Q.C °úÁ¤ÀÌ ´õ¿í ºü¸£°í Æí¸®ÇØÁú °ÍÀÌ´Ù. ±×·¯ÇÏ¿© PDA UV-Vis SpectrophotometerÀÇ ÀÌ·± Ư¡Àº »ý»ê¼º Çâ»ó¿¡ Á÷°áµÉ °ÍÀÌ´Ù.
°íü SampleÀÇ Åõ±¤µµ´Â ÀԻ簢µµ¿¡ µû¶ó ´Þ¶óÁú ¼ö ÀÖÀ¸¹Ç·Î, À̹ø ½ÇÇè¿¡¼´Â SampleÀ» Magnetic Film Holder¿¡ ¼öÁ÷À¸·Î °íÁ¤½ÃÄÑ ÀçÇö¼º ÀÖ°í Á¤È®ÇÑ Data¸¦ ¾òÀ» ¼ö ÀÖ¾ú´Ù.
Reagents & Apparatus
1. S-3150 (PDA UV-Vis Spectrophotometer)
2. Magnetic Film Holder Accessory
3. LabPro¢ç Plus S/W
4. Sample (Glass Plate, IR Filter)
Procedure
1. Parameter¸¦ ¼³Á¤ÇÑ´Ù.
2. Blank¸¦ ÃøÁ¤ÇÑ´Ù.
3. Magnetif Film Holder¿¡ Sample
À» °íÁ¤½ÃŲ´Ù.
4. Åõ±¤µµ¸¦ ÃøÁ¤ÇÑ´Ù.
Instrument Parameters
Àû¿ëÇÑ Parameter´Â ´ÙÀ½°ú °°À¸¸ç, Figure 1¿¡ Ãø¸é ȸéÀ» ³ªÅ¸³»¾ú´Ù.
Experiment setup
Data Type : Transmittance
Sampling : Single Cell
Spectra No. : 1
Scan No. : 30
Integration No. : 1
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